rabbit anti aplnr  (Proteintech)

Journal: iScience

Article Title: Elabela inhibits TRAF1/NF-κB induced oxidative DNA damage to promote diabetic foot ulcer wound healing

doi: 10.1016/j.isci.2023.107601

Figure Lengend Snippet:

Article Snippet: rabbit anti-apelin receptor , Proteintech , Cat# 20341-1-AP, RRID: AB_2878676.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software, Extraction

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Immunostaining for APLN, APLNR and serotonin. Positivity to APLN ( a ) and APLNR ( d ) are localized in the epithelium of the abomasum. Fundic glands are positive to APLN ( b ) and APLNR ( e ); each inset shows a magnification of the corresponding image. Double-label immunohistochemistry shows serotonin-positive neuroendocrine cells (blue stained) that are distinct from APLN- ( c ) and APLNR- ( f ) positive cells (brown stained).

Article Snippet: For the immunohistochemical reaction, sections were incubated overnight at room temperature (RT) with a 1:200 rabbit polyclonal anti-APLN antibody (Novus Biological, Littleton, CO, USA), a 1:400 rabbit polyclonal anti-APLNR antibody (Abnova, Taipei City, Taiwan) and a 1:150 rabbit polyclonal anti-APLNR antibody (Novus Biological, Littleton, CO, USA).

Techniques: Immunostaining, Immunohistochemistry, Staining

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Immunostaining for APLNR in the duodenum. Positivity to APLNR is shown in the epithelium ( a ), intestinal crypts (*) and neuroendocrine cells (arrows; ( b , c )), while duodenal glands (arrowhead) appear negative ( b ). A higher magnification of an intestinal crypt (*) with a positive neuroendocrine cell (arrow) is shown ( c ).

Article Snippet: For the immunohistochemical reaction, sections were incubated overnight at room temperature (RT) with a 1:200 rabbit polyclonal anti-APLN antibody (Novus Biological, Littleton, CO, USA), a 1:400 rabbit polyclonal anti-APLNR antibody (Abnova, Taipei City, Taiwan) and a 1:150 rabbit polyclonal anti-APLNR antibody (Novus Biological, Littleton, CO, USA).

Techniques: Immunostaining

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Immunofluorescence for APLNR (( a ), green) and serotonin (( b ), red) in the intestinal crypts of the duodenum. Colocalization of APLNR and serotonin is showed in neuroendocrine cells (( c ), merge).

Article Snippet: For the immunohistochemical reaction, sections were incubated overnight at room temperature (RT) with a 1:200 rabbit polyclonal anti-APLN antibody (Novus Biological, Littleton, CO, USA), a 1:400 rabbit polyclonal anti-APLNR antibody (Abnova, Taipei City, Taiwan) and a 1:150 rabbit polyclonal anti-APLNR antibody (Novus Biological, Littleton, CO, USA).

Techniques: Immunofluorescence

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Responses by immunoreactive structures to APLN and APLNR detection treatments in the three different animal groups, expressed as mean intensity values.

Article Snippet: For the immunohistochemical reaction, sections were incubated overnight at room temperature (RT) with a 1:200 rabbit polyclonal anti-APLN antibody (Novus Biological, Littleton, CO, USA), a 1:400 rabbit polyclonal anti-APLNR antibody (Abnova, Taipei City, Taiwan) and a 1:150 rabbit polyclonal anti-APLNR antibody (Novus Biological, Littleton, CO, USA).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Statistical significance of differences ( p < 0.01) for each histochemical treatment among different animal groups, as performed by Kruskal–Wallis tests, and respective pairwise comparisons, as performed by Wilcoxon rank sum tests. p -values were adjusted for multiple testing using the Holm correction. Significant values are indicated in bold.

Article Snippet: For the immunohistochemical reaction, sections were incubated overnight at room temperature (RT) with a 1:200 rabbit polyclonal anti-APLN antibody (Novus Biological, Littleton, CO, USA), a 1:400 rabbit polyclonal anti-APLNR antibody (Abnova, Taipei City, Taiwan) and a 1:150 rabbit polyclonal anti-APLNR antibody (Novus Biological, Littleton, CO, USA).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Immunostaining for APLN, APLNR and serotonin. Positivity to APLN ( a ) and APLNR ( d ) are localized in the epithelium of the abomasum. Fundic glands are positive to APLN ( b ) and APLNR ( e ); each inset shows a magnification of the corresponding image. Double-label immunohistochemistry shows serotonin-positive neuroendocrine cells (blue stained) that are distinct from APLN- ( c ) and APLNR- ( f ) positive cells (brown stained).

Article Snippet: Then, they were incubated with normal goat serum for 1 hour and a half and incubated overnight with a 1:500 rabbit anti-APLNR antibody (Novus Biological, Littleton, CO, USA) and with a 1:500 mouse anti-serotonin antibody (M0758, DakoCytomation, Glostrup, Denmark).

Techniques: Immunostaining, Immunohistochemistry, Staining

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

doi: 10.3390/ani11113173

Figure Lengend Snippet: Immunofluorescence for APLNR (( a ), green) and serotonin (( b ), red) in the intestinal crypts of the duodenum. Colocalization of APLNR and serotonin is showed in neuroendocrine cells (( c ), merge).

Article Snippet: Then, they were incubated with normal goat serum for 1 hour and a half and incubated overnight with a 1:500 rabbit anti-APLNR antibody (Novus Biological, Littleton, CO, USA) and with a 1:500 mouse anti-serotonin antibody (M0758, DakoCytomation, Glostrup, Denmark).

Techniques: Immunofluorescence